Comparison of the hormonal induction of spawning by one or two primings
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nDay 1. Ten female toads (two primings) were injected with hormones (500IU of human chorionic gonadotropin (hCG)).
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nDay 3. The original ten plus ten new toads (one priming) were injected with hormones (100IU hCG and 0.8pg LHRHa).
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nSpawning injection (500IU hCG and 4pg LHRHa).
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nSpawning was independent of the males and into a solution (simplified amphibian ringers) which maintained the viability of the oocytes for two hours.
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