•Action 3.1: Trial the real-time PCR test for
chytridiomycosis in a field situation and compare for sensitivity against the histological examination of toe clips.
•Action 3.2: Evaluate pooling samples as a cost
effective technique for
surveying large numbers of animals using the real-time PCR test, with subsequent testing
of individual samples in positive pools.
•Action 3.6: Develop criteria for a wide-scale survey
protocol applicable at
the national level and carry out a survey in chytrid-free and chytrid-contaminated areas
and evaluated by comparison with sites that are known to be infected with chytridiomycosis.
• Action 3.7: To facilitate the rapid detection of B. dendrobatidis using PCR, establish a national quality accredited laboratory dedicated to the
diagnosis of chytridiomycosis.
•