Diagnostic technique: issues
•Low sensitivity
•Nationwide chytrid survey proposed by TAP: determine chytrid-status of many populations nationwide.
•Q: How many frogs must be sampled from each population?
•Number of animals to be sampled in order to be 95% confident that chytrid is ABSENT from a population relies on knowledge of expected prevalence rate in that population. (DiGiacomo and Koepsell 1986)