Comparing Histological with Real-time Taqman PCR

Taqman PCR/Swabs vs. Histology/Toe-Clips

Ø 104 wild Mixophyes iteratus near Peachester, SEQ

• Every frog both swabbed (PCR) and toe-clipped (Histo).

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Ø Population chosen because

• Site historically chytrid positive.

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• Frogs already being used for mark-recapture study.

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• Lowland site…light infections expected.

v Light infections would pronounce the inadequacies of an insensitive detection technique.

v Heavy infections likely to be detected even by insensitive technique, resulting in a Type II error.


	Light infections xpected based on no declines, and low altitude. Type 2: falsely concluding that there is no difference in techniques, when there is.