Real-time Taqman PCR on
toe-clips (Boyle et al. 2004)
ØImprovements over
histology…
•Sensitive:
•Detects
chytrid 7 to 14 days prior to histo; nearly twice as likely to detect chytrid than is IPX (Boyle et al. 2004)
•Specific:
•Minimal
chance of false positives.
•Quantitative:
•Can
detect and quantify a single chytrid zoospore.
•Rapid:
•80
samples per day
