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Histology
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H&E stain
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1 to 3 toes examined per frog (mean = 2.3)
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Roughly 5 sections per toe examined.
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Real-time Taqman PCR
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Swabs frozen upon return from field.
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Analysed in triplicate.
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Methods
H&E chosen because accuracy not much less than IPX, and since it has been the most widely used diagnostic technique in the past, this study will allow us to judge by how much those studies may have under-estimated prevalence.
