Amphibian Research Group, Univ of
Newcastle
NUCLEAR
TRANSFER IN AMPHIBIANS
The egg is enucleated physically or with ultraviolet light and is used
as a donor egg (cytoplast). The cytoplast is then microinjected with a
single donor nucleus (karyoplast) from an adult somatic or
embryonic source.
Potentially – the donor cells could
be from one species and the egg from
another,
allowing for the possibility of
“resurrecting”
extinct species if the frozen material is
available.