Amphibian Research Group, Univ of
Newcastle
Why can’t we freeze amphibian eggs?
• maybe we can, but we think we can’t because
no-one
has
reported successful freezing of fish eggs
which
are like frogs’ eggs in many respects
Zebra fish egg
• egg is too large to
cool rapidly during
freezing
• yolk freezes poorly
•Barriers to cryoprotectant
penetration (YSL)
>
1 mm
Hagedorn et al, 1997
Mammal’s
Egg/embryo