Amphibian Research Group, Univ of
Newcastle
Generic
Protocol for Amphibian Sperm Cryopreservation
High diluent osmolality
(> 200 mosm kg -1)
- sucrose preferable to inorganic electrolytes
- sucrose preferable to inorganic electrolytes
High cryoprotectant
concentration: Me2SO preferable to glycerol
Slow cooling/freezing rate
Thaw at room temperature
From: Browne, Clulow,
Mahony, Clark 1998 Cryobiology 37: 339-345